Biobase Fluorescent Quantitative PCR Dtection System PCR Machine

• Customization: Available
• Classification: Biochemical Apparatus
• Type: Fluorescent Quantitative PCR Dtection System
• Certification: CE, ISO13485
• Group: All
• Model: Bh-Ny01s
• Sample Capacity: 96*0.1ml PCR Plate
• Reaction System: 10~50μl
• Dynamics Range: 1~1010 Copies
• Channel: 4
• Emission Light: LED
• Detector: Mppc
• Detection Path: F1
• Excitation Wavelength: 455~680nm
• Detection Wavelength: 510~730nm
• Fluorescence Detection Repeatability: CV≤2%
• Fluorescence Detection Accuracy: CV≤3%
• Fluorescence Detection Linearity: R≥0.995
• Transport Package: Standard Export Packaging
• Specification: 645*565*605
• Trademark: BIOBASE
• Origin: China
• Production Capacity: 100/Year

Product Description

Fluorescent Quantitative PCR Dtection System

Introduction:
Real-time PCR is used for sensitive, specific detection and quantification of nucleic acid targets. We have developed powerful assay design algorithms, optimized qPCR regent, intuitive data analysis software, and flexible instrumentation to help harness the power of qPCR across a rich and diverse set of applications. Explore our robust solutions for your qPCR-based research.

Application:

It can be widely used for Infectious disease research, Food pathogen detection, Waterborne pathogen detection, Stem cell research, Oncology and genetic disease research, Plant sciences and agricultural biotechnology.

 

Working Principle:

The temperature step change is controlled by the semiconductor peltier to realize PCR amplification. Use high-sensitivity PD unit to detect fluorescence. Program control channel switching, non-contact excitation/detection on the top structure, coordinated with motor control X and Y axis movement to achieve 96-hole scanning. Finally, accurate analysis is carried out through powerful software.

 

 

 

Features:

1. Excellent temperature control performance of the instrument, Module Max heating rateheating rate 7.0ºC/s.

2. No edge effect, no optical path correction, top excitation/detection, non-contact measurement.

3. Fluorescence detection adopts PD sensor with high sensitivity.

4. Long-life LED light source, stable emission wavelength, maintenance-free.

5. 4/6-channel fluorescence detection, no cross interference between channels.

6. User-friendly and fully functional software, flexible program setting, comprehensive analysis and reporting functions, all the parameters can be stored.

 

Real-time PCR Hardware:

 

 

Thermoelectric cooling module(TEM) is a semiconductor device composed of many tiny and effective heat pumps. By applying a low-voltage DC power supply, heat will be transferred from one side of the TEM to the other side, resulting in a phenomenon that one side of the TEM becomes hot and the other side becomes cold. Since this phenomenon is completely reversible, when the polarity of the DC power supply is changed, it will be affected. Shift in the opposite direction. This product adopts a long-life series TEM, which provides longer life and more efficiency during thermal cycling.

 

The integrated design of the scanning module and the heating cover module, relying on its own gravity to compress the heating plate and the reagent cover, and is supported by four compression springs to prevent the sample tube from being crushed. At the same time, the rubber pad around the heating cover is pressed to ensure that there is no external light source interference in the detection. The bottom of the cam mechanism is used to support the spacing to ensure the smooth sliding of the heating module. The guide rail mechanism on both sides of the heating module prevents the module from shifting and ensures the accuracy of the mechanical scanning structure.

 

Real-time PCR Software:

 

The software includes functions such as absolute quantification experiment, melting curve experiment, relative quantification (AACT) experiment, and genotyping experiment. Enter the attribute setting interface and select different function modules. Guided flow operation, convenient for users to quickly complete experimental settings. The software can open the recent experiment record template for easy viewing of recent experiments and the creation of new experiments.

Model	LEIA-X4
Sample Capacity	96*0.1ml PCR plate, 12*8-strip tubes, 96*0.1ml single tube(Transparent cover)
Reaction System	10~50μl
Dynamics Range	1~10^10 copies
Channel	4
Emission Light	LED
Detector	MPPC
Detection Path	F1	F2	F3	F4
Suitable Probe/Dye	FAM/SYBR GREEN	VIC/JOE/ HEX/TET	ROX/TEXAS-RED	Cy5
Excitation Wavelength	455~680nm
Detection Wavelength	510~730nm
Fluorescence Detection Repeatability	CV≤2%
Fluorescence Detection Accuracy	CV≤3%
Fluorescence Detection Linearity	r≥0.995
Module Temp. Range	4~99ºC(Resolution: 0.1ºC)
Ramp Rate	5.0ºC/s(Max)
Temp. Accuracy	±0.3ºC
Temp. Uniformity	≤±0.3ºC
Temp. Control Mode	Block mode
Gradient Temp. Range	1~36ºC
Hot-lid Temp. Range	100ºC, automatic hot-lid
Scanning Mode	Full plate scanning
Programming	Max 100 segments for each program, max 99 cycles
Operation Mode	Continuous
Scanning Time	8.5s
Special Function	Absolute quantitative automatic analysis, relative quantification, SNP analysis, melting curve analysis, etc
Operation System	Microsoft: Windows10
Power Supply	220V, 50/60Hz; 110V, 60Hz
Dimension(L*W*H)mm	375*490*365
Port Method	USB Port
Packing Size(L*W*H)mm	645*565*605
Gross Weight(kg)	45

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