Quantitive PCR machine/qPCR detective equipment
Parameter details:
| THERMAL BLOCK |
| Block formats |
96-well |
| Sample Volume |
5-50ml |
| Consumables |
0.1ml 96-well unskirted Plates; 0.1ml 8-strip tubes (Optical flat cap) |
| Max heating/cooling rate |
4.2ºC/sec |
| Temperature Range |
4.0-99.0ºC |
| Temperature Accuracy |
≤0.1ºC |
| Temperature Uniformity |
<0.3ºC |
| Temperature Steps |
1-99 |
| HEAT UP |
| Temperature Range |
100-120ºC |
| Control |
Automatic temperature and pressure setting |
| OPTICS |
| Excitation |
4LEDs |
| Excitation range |
470-630nm |
| Detector |
Photodiode |
| Detection Range |
525-670nm |
| Detectoin Channels |
| Channel |
Exciation(nm) |
Emission (nm) |
Pre-Calibrated Dyes |
| 1 |
470 |
525 |
FAM, SYBR Green I |
| 2 |
523 |
564 |
VIC, HEX, TET, JOE |
| 3 |
571 |
621 |
ROX, TEXAS-REO |
| 4 |
630 |
670 |
Cy5 |
| Multiplex |
Up to 4 targets |
| Detection Range |
1-1010 copies |
| SOFTWARE |
|
PERFORMANCE |
|
| Analysis modes |
Absolute quantification, relative quantification |
Reproducibility |
CV<2.1% |
| Melt Curve Analysis, High Resolution Melting |
Correlation Coefficient of nucleic acid precision |
G>0.997 |
| SYSTEM |
| Operating Systems |
Windows XP, Win7 |
| Communication |
Ethemet or USB |
| Power usage |
AC220*(1+-10%)V, 50*(1+-2%)Hz |
| Dimensions |
540W*300D*480H mm |
| Weights |
35kg |
Design for Flexible use
The BIO-EII System adapts to almost any workflow requirement with flexible instrument control and data management.
- Standard with 7" LCD touch screen, functions quickly accessible through simple, one-touch commands.High-throughput up to 96 samples per run.Push-pull, electrical controlled elevating platform.Concealed lid with constant temperature and isopiestic pressure bring high accurate temperature controlQuick-start set-up so you can begin a run immedicately.Unique power-off protection function can save all the setted configurations for sudden power outages, and allow the experiment continues when power supply is restored.System can be installed in three distinct configurations, providing unmatched flexibiity and convenience.
A. Co-located configuration (PC control)
B. Standalone (PC free) configuration (PC software automatically detect all systems in
LAN and allow to remote monitoring one of experimental progress and downloading
of the completed run file to the PC at your desk)
Fast and uniform heating and heat sinking block
- Concealed lid with constant temperature and isopiestic pressureElectrical controlled elevating & automatic locked platformThermo block is based on improved heat pipe technology, leading to extraordinary lintra-well temperature homogeneity and maximized inter-well, inter-cycle reproducibiity.Highly efficient PID & DSP control algorithm ensures faster respond and stronger interference resistance, fast and uniform heating and heat sinking.
High sensitive and precise optical system
- High-sensitivity and wide dynamic range of fluorescence detection system, sample dynamic range could e from 1 to 1010 copies.Fast and precise mechanica scanning struture ensures the illumination uniformity of 96 wells, eliminate edge effects, no need for ROX calibrationFluorescence excitation by a high-intensity and long-life LED, Bulb Luminance will not attenuation over time, free maintenance optical system through the entire lifeProfessional designed fluorescence filters realize the highly fluorescence transmittance and meanwhile minimize the fluorescence channel crosstalk.
Easy-to-use software
The BKTL988-IV Software offers an intuitive experience for protocols programming plate layouts setting; and experimental results analysising, The Soft ware includes an unlimited license for multiple users, the avaiable software modules include Absolute quantification, Melting curve analyssm Relative quantification and High Resolution Melting (HRM). All the excellent features mae the instrument to your ideal choice for qPCR.
- Friendly interface and clearly functional modules design, first-time uses can easily start a new experiment.All experimet date and setting options are shown conveniently on a single interface. User modifications to analysis parameters can be seen instantly.Powerful analytical capabilities, optional automatic and manual threshold setting bring reliable data you can work on.Experimental datas can be export in .csv, excel, .txt formats, with this capability. The BIO-EII can interface with your laboratory information system directly.Unique designed universal report can be customized according to the requirements, report review an print function help clinical documetation as needed.
Generate the quality date that you can depend on:
This real-time PCR run was done using 5ng of hepatitis B virus DNA in each of the 96 block positions. (A) A 135 bp amplicon of the hepatitis B virus gene was amplified using SYBR Green I detection. (B) The reaction product was also subjected to melting curve analysis. In summay, low variation Cq values (Cq range = 0.15 and SD=0.040) and overlapping melt curves of the amplicon (Tm range of 0.5ºC and SD= 0.065) in each of the 96 positions demonstrated temperature homogeneity and equal treatment of all samples-independent of block position.
Dynamic range of Gene Quantification at low dilutions:
A hepatitis B virus gene fragment was amplified in ten serial 1:5 dilutions ranging from 108-1010 copies per well. Eight replicates were run for each dilution. Results on the BIO-EII System show excellent reproducibility and resolution down to very low copy numbers.
Certificates:
Biobase show:
Package & delivery:
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